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Biology · 2024 · 3 marks
CBSE 2024 · Region 1 · Set 1 · Q27
(a) (i) What are transgenic animals? (ii) Name the first transgenic cow and state its importance.
(a)(i)
What are transgenic animals ?
(ii)
Name the first transgenic cow and state its importance.
OR
(b)(i)
Explain the convention for naming EcoRI.
(ii)
With the help of an illustration only, show the action of EcoRI on a DNA Polynucleotide.
Marking-scheme solution
(i)
Transgenic animals are those which have their DNA manipulated to possess and express a
foreign gene.
(ii)
First transgenic Cow – Rosie,
-Produced human protein enriched milk ($\displaystyle 2$⋅$\displaystyle 4$ g/litre) / cow milk containing human alpha
lactalbumin protein is nutritionally more balanced product for human babies than natural cow milk.
(i)
In EcoRI (comes from Escherichia coli RY13)
-E represent Genus Escherichia ,
-co represent species coli,
-R represent RY $\displaystyle 13$ strain,
-I represent order in which the enzyme were isolated from that strain of bacteria.
(ii)
Vector DNA and Foreign DNA are each cut by EcoRI at GAATTC, producing complementary sticky ends.
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