CBSE 2025 · Region 6 · Set 2 · Q32 · 5 marks
(a)Describe the approach of gene therapy used for the treatment of a $\displaystyle 4$-year-old girl suffering from Adenosine Deaminase Deficiency.(ii)What is meant by microprogation ? Name any two important food plants grown commercially (on a large scale) by this method.Describe the technique of a typical agarose gel electrophoresis used for the separation and isolation of DNA fragments.
(a)
Describe the approach of gene therapy used for the treatment of a $\displaystyle 4$-year-old girl suffering from Adenosine Deaminase Deficiency.
(ii)
What is meant by microprogation ? Name any two important food plants grown commercially (on a large scale) by this method.
Describe the technique of a typical agarose gel electrophoresis used for the separation and isolation of DNA fragments.
Marking-scheme solution
(a)
Lymphocytes from the blood of the patient are grown in a culture outside the
body, a functional ADA cDNA (using a retroviral vector) is then introduced into these lymphocytes, which are subsequently returned to the patient.
(ii)
Method of producing thousands of plants through tissue culture is called micro-
propagation, Used in Tomato/Banana/Apple
•
DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix, the DNA fragments separate (resolve) according to their size through sieving effect, smaller DNA fragment move farther
•
-The separated DNA fragments can be visualized after staining the DNA
with a compound known as ethidium bromide followed by exposure to UV radiation, The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
Biotechnology and its ApplicationsBiotechnological Applications in MedicineUnderstandlong_answerhard
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CBSE Class 12 Biology past-paper question from the 2025board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.