(a) (i) Describe the approach of gene therapy used for the treatment of a 4-year-old girl suffering from Adenosine Deaminase Deficiency. (ii) What is…

Lymphocytes from the blood of the patient are grown in a culture outside the

body, a functional ADA cDNA (using a retroviral vector) is then introduced into these lymphocytes, which are subsequently returned to the patient.

Method of producing thousands of plants through tissue culture is called micro-

propagation, Used in Tomato/Banana/Apple

• DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix, the DNA fragments separate (resolve) according to their size through sieving effect, smaller DNA fragment move farther

• -The separated DNA fragments can be visualized after staining the DNA

with a compound known as ethidium bromide followed by exposure to UV radiation, The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.