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Biology · 2022 · 3 marks
CBSE 2022 · Region 4 · Set 1 · Q10
With the help of a storyboard or a flowchart only, explain all the stages of how human insulin is produced through r-DNA technology.
With the help of a storyboard or a flowchart only, explain all the stages of how human insulin is produced through r-DNA technology.
Marking-scheme solution
Preparation of two DNA sequences corresponding to A, and B chains of human insulin (using rDNA technology)
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Introduction in plasmids of E. coli
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Chain A and B were separately produced
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Chain A and B were extracted
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Both chains were combined by creating di-sulphide bonds to form human insulin.
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