CBSE 2022 · Region 4 · Set 1 · Q10 · 3 marks
With the help of a storyboard or a flowchart only, explain all the stages of how human insulin is produced through r-DNA technology.
Marking-scheme solution
Preparation of two DNA sequences corresponding to A, and B chains of human insulin (using rDNA technology)
Introduction in plasmids of E. coli
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Chain A and B were separately produced
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Chain A and B were extracted
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Both chains were combined by creating di-sulphide bonds to form human insulin.
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CBSE Class 12 Biology past-paper question from the 2022board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.