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Biology · 2025 · 2 marks
Biotechnology and its ApplicationsBiotechnological Applications in Medicine2 marksUnderstandvery short answer
CBSE 2025 · Region 1 · Set 1 · Q21
Student to attempt either option-(A) or (B): (A) A patient with ADA deficiency requires periodic infusion of genetically engineered lymphocytes.…
Student to attempt either option-(A) or (B) :
(A)
A patient with ADA deficiency requires periodic infusion of genetically engineered lymphocytes. Explain why such periodic infusion is required and also suggest a permanent cure for such ADA deficiency.
OR
(B)
Describe in brief any two techniques that can be utilised to transfer recombinant DNA into the host cell directly without using any vector.
Marking-scheme solution
The genetically engineered lymphocytes are not immortal hence the patient requires periodic infusion
There could be permanent cure if the gene isolated from bone marrow cells producing ADA is introduced into cells (lymphocytes) at early embryonic stages
Micro-injection, Recombinant DNA is directly injected into the nucleus of an animal/
Biolistics or gene gun, plants cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA/
Heat shock , Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice which is followed by placing them briefly at 42o C (heat shock) and then putting them back on ice.
(Any Two Techniques)
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CBSE Class 12 Biology past-paper question from the 2025 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.