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Biology · 2022 · 3 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology3 marksApplyshort answer
CBSE 2022 · Region 2 · Set 1 · Q9
With the advent of sophisticated techniques of genetic engineering, we can now readily purify and isolate DNA. Name and explain the different steps…
With the advent of sophisticated techniques of genetic engineering, we can now readily purify and isolate DNA. Name and explain the different steps involved in the separation and isolation of DNA fragments once cut by restriction endonucleases.
Marking-scheme solution
Gel electrophoresis
Negatively charged DNA fragments (produced by restriction endonuclease) move towards anode through agarose gel, smaller fragments move further, separated fragments are stained with ethidium bromide, followed by exposure to UV radiation, extraction of DNA bands by elution.
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