✓ Board-verified
Biology · 2022 · 3 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology3 marksUnderstandshort answer
CBSE 2022 · Region 5 · Set 2 · Q10
Explain how recombinant human insulin was prepared in 1983 by Eli Lily an American company.
Explain how recombinant human insulin was prepared in $\displaystyle 1983$ by Eli Lily an American company.
Marking-scheme solution
Two DNA sequences corresponding to chain A, and chain B of human insulin were prepared using rDNA technology, Introduced separately in plasmids of E. coli to produce insulin chains, Chains A and B were separately produced, Extracted the chains, Combined by creating disulphide bonds to form human insulin
All Biotechnology : Principles and Processes questions2022 question paperNCERT solutions: Biotechnology : Principles and ProcessesPractice Biotechnology : Principles and Processes →
More from Biotechnology : Principles and Processes
- Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule. 5^′ C-T-G-C-A ↓- G 3^′; 3^′ G ↑-…2023 · asked 2×
- The linking of the antibiotic resistance gene with the plasmid vector of Salmonella typhimurium by Stanley Cohen and Herbert Boyer was made possible…2024
- Which of the following is not required for PCR?2024
- With reference to the set-ups (A, B and C) given below, of the electrophoretic separation of a mixture of DNA fragments of varied lengths, answer the…2024
- (i) Given below is the schematic representation of the process of electrophoresis. Identify the alphabets representing the: (I) Anode end, and (II)…2026
- Assertion: The stirrer facilitates the even mixing of oxygen availability in a bioreactor. Reason (R): Stirred-tank bioreactors generally have a flat…2024
- The organism used in construction of the first artificial recombinant DNA by Cohen and Boyer in 1972 was:2023
- How is DNA isolated in purified form from a bacterial cell? OR Explain how human functional insulin was produced by using recombinant DNA (rDNA)…2025
CBSE Class 12 Biology past-paper question from the 2022 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.