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Biology · 2022 · 3 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology3 marksUnderstandshort answer
CBSE 2022 · Region 5 · Set 1 · Q9
A cell free method of amplifying DNA first developed in the mid 1980's revolutionized the field of biotechnology. Name the method and explain the…
A cell free method of amplifying DNA first developed in the mid $\displaystyle 1980$'s revolutionized the field of biotechnology. Name the method and explain the basic steps of the technique involved.
Marking-scheme solution
-Polymerase Chain Reaction / PCR
-Denaturation by heating /DNA strands are separated by heating.
Annealing of two primers to complementary region of DNA/ Joining of primer to complementary region of DNA
Extension of primers ,using thermostable DNA Polymerase or Taq Polymerase .
(The process is repeated many times/amplification)
-Polymerase Chain Reaction/PCR
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CBSE Class 12 Biology past-paper question from the 2022 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.