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Biology · 2022 · 3 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology3 marksApplyshort answer
CBSE 2022 · Region 3 · Set 2 · Q7
Restriction endonucleases are used in genetic engineering to form recombinant DNA. Explain only with the help of a flow chart the steps carried in…
Restriction endonucleases are used in genetic engineering to form recombinant DNA. Explain only with the help of a flow chart the steps carried in the formation of a recombinant DNA.
Marking-scheme solution
Restriction endonuclease cuts both DNA (vector and foreign DNA) at the same site (a little away from the centre of palindrome sites)
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Leaves single stranded portion at the ends of each DNA strand / formation of sticky ends on each DNA strand
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DNA fragments join at sticky ends by DNA ligase to form rDNA.
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Action of Restriction enzyme
DNA fragments join at sticky ends
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DNA fragments join at sticky ends
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