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Biology · 2024 · 3 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology3 marksApplyshort answer
CBSE 2024 · Region 4 · Set 2 · Q26
The schematic representation as above is showing the linking of two DNA fragments. (a) Name 'A' and 'B' fragments. (b) Write the 'palindrom'…
The schematic representation as above is showing the linking of two DNA fragments.
(a)
Name 'A' and 'B' fragments.
(b)
Write the 'palindrom' recognised by EcoRI.
(c)
Where does EcoRI cut the palindrome ? Write the events followed thereafter to form a recombinant DNA.
Marking-scheme solution
(a)
• A– vector DNA,
• B is foreign DNA/ Desired DNA/ Alien DNA
(b)
(c)
It cuts the DNA between bases G and A only , It give rise to sticky ends of
‘A’ DNA and ‘B’ DNA which is joined by ligase enzyme to form recombinant DNA.
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CBSE Class 12 Biology past-paper question from the 2024 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.