Read the passage given below and answer the questions that follow. In recombinant DNA technology, restriction enzymes are used as they recognize and…

Read the passage given below and answer the questions that follow. In recombinant DNA technology, restriction enzymes are used as they recognize and cut DNA within a specific recognition sequence. BamH I is one such restriction enzyme which binds at the recognition sequence $\displaystyle 5$' G-G-A-T-C-C $\displaystyle 3$' and cleaves this sequence between G and G on each strand, whereas Alu I binds at the recognition sequence $\displaystyle 5$' A-G-C-T $\displaystyle 3$' and cleaves these sequences between G and C on each strand.

If Alu I is used to cut the given DNA strand, how many DNA fragments would be formed ? Write the sequence of each fragment formed with its polarity.

Which one of the two restriction enzymes BamH I or Alu I will preferably be used on the same given DNA strand to make a recombinant DNA molecule and why ?

After binding to the two strands of the double helix DNA, where specifically does the restriction enzyme act to cut the two strands of DNA ? Write the specific term used for the specific nucleotide sequences of DNA recognised by a restriction endonuclease.

Write the specific sequence of DNA segment recognised by the restriction endonuclease EcoRI.