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Biology · 2024 · 4 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology4 marksRemembercase study
CBSE 2024 · Region 1 · Set 1 · Q30
Study the diagram given below that shows the steps involved in the procedure of selecting transformed bacteria and answer the questions that follow:…
Study the diagram given below that shows the steps involved in the procedure of selecting transformed bacteria and answer the questions that follow :
(a)
Identify the colony that has got transformed. Justify your answer.
(b)
What are the sites in a plasmid called where ampicillin and tetracycline resistance genes are inserted ? State their role in genetic engineering.
(c)
Name two enzymes playing an important role in genetic engineering.
OR
(c)
State the role of $\displaystyle \beta$-galactosidase in insertional inactivation.
Marking-scheme solution
(a)
Colony $\displaystyle 4$ is transformed with plasmid containing recombinant DNA, as they will not show
resistance towards tetracycline.
(b)
[Official scheme awards full credit for this part; no model answer is printed.]
(c)
Restriction endonuclease / ligase / Taq DNA Polymerase
OR
(c)
Insertional inactivation of gene encoding for ß- galactosidase will lead to colorless
bacterial colonies (recombinant)
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CBSE Class 12 Biology past-paper question from the 2024 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.