✓ Board-verified
Biology · 2024 · 5 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology5 marksApplylong answer
CBSE 2024 · Region 5 · Set 1 · Q31
(a) (i) Draw a schematic diagram of the cloning vector pBR 322 and label (1) Bam HI site (2) gene for ampicillin resistance (3) 'ori' (4) 'rop' gene.…
(i)
Draw a schematic diagram of the cloning vector pBR $\displaystyle 322$ and label ($\displaystyle 1$) Bam HI site ($\displaystyle 2$) gene for ampicillin resistance ($\displaystyle 3$) 'ori'
(4)
'rop' gene.
(ii)
State the role of 'rop' gene.
(iii)
A cloning vector does not have a selectable marker. How will it affect the process of cloning ?
(iv)
Why is insertional inactivation preferred over the use of selectable markers in cloning vectors ?
(i)
Name the nematode (scientific name) that infects the roots of tobacco plant and reduces its yield.
(ii)
Name the vector that is used to introduce nematode-specific genes into the host plant (tobacco).
(iii)
How do sense and anti-sense RNAs function ?
(iv)
Why could parasite not survive in a transgenic tobacco plant?
Marking-scheme solution
(i)
(ii)
‘rop’ codes for proteins involved in the replication of the plasmid.
(iii)
It will not be possible to differentiate transformants from the non-
transformants or to differentiate recombinants from non-recombinants.
(i)
Meloidegyne incognitia
(ii)
Agrobacterium tumefaciens / Ti Plasmid
(iii)
Both sense and antisense RNA are complementary to each other, form a
double stranded RNA (dsRNA)
(iv)
The double stranded RNA binds to a specific mRNA / initiate RNAi ,
Prevents translation of mRNA / silencing of specific mRNA of parasite or nematode.
All Biotechnology : Principles and Processes questions2024 question paperNCERT solutions: Biotechnology : Principles and ProcessesPractice Biotechnology : Principles and Processes →
More from Biotechnology : Principles and Processes
- Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule. 5^′ C-T-G-C-A ↓- G 3^′; 3^′ G ↑-…2023 · asked 2×
- The linking of the antibiotic resistance gene with the plasmid vector of Salmonella typhimurium by Stanley Cohen and Herbert Boyer was made possible…2024
- Which of the following is not required for PCR?2024
- With reference to the set-ups (A, B and C) given below, of the electrophoretic separation of a mixture of DNA fragments of varied lengths, answer the…2024
- (i) Given below is the schematic representation of the process of electrophoresis. Identify the alphabets representing the: (I) Anode end, and (II)…2026
- Assertion: The stirrer facilitates the even mixing of oxygen availability in a bioreactor. Reason (R): Stirred-tank bioreactors generally have a flat…2024
- The organism used in construction of the first artificial recombinant DNA by Cohen and Boyer in 1972 was:2023
- How is DNA isolated in purified form from a bacterial cell? OR Explain how human functional insulin was produced by using recombinant DNA (rDNA)…2025
CBSE Class 12 Biology past-paper question from the 2024 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.