CBSE 2024 · Region 5 · Set 1 · Q31 · 5 marks
(i)Draw a schematic diagram of the cloning vector pBR $\displaystyle 322$ and label ($\displaystyle 1$) Bam HI site ($\displaystyle 2$) gene for ampicillin resistance ($\displaystyle 3$) 'ori'(4)'rop' gene.(ii)State the role of 'rop' gene.(iii)A cloning vector does not have a selectable marker. How will it affect the process of cloning ?(iv)Why is insertional inactivation preferred over the use of selectable markers in cloning vectors ?(i)Name the nematode (scientific name) that infects the roots of tobacco plant and reduces its yield.(ii)Name the vector that is used to introduce nematode-specific genes into the host plant (tobacco).(iii)How do sense and anti-sense RNAs function ?(iv)Why could parasite not survive in a transgenic tobacco plant?
(i)
Draw a schematic diagram of the cloning vector pBR $\displaystyle 322$ and label ($\displaystyle 1$) Bam HI site ($\displaystyle 2$) gene for ampicillin resistance ($\displaystyle 3$) 'ori'
(4)
'rop' gene.
(ii)
State the role of 'rop' gene.
(iii)
A cloning vector does not have a selectable marker. How will it affect the process of cloning ?
(iv)
Why is insertional inactivation preferred over the use of selectable markers in cloning vectors ?
(i)
Name the nematode (scientific name) that infects the roots of tobacco plant and reduces its yield.
(ii)
Name the vector that is used to introduce nematode-specific genes into the host plant (tobacco).
(iii)
How do sense and anti-sense RNAs function ?
(iv)
Why could parasite not survive in a transgenic tobacco plant?
Marking-scheme solution
(i)
(ii)
‘rop’ codes for proteins involved in the replication of the plasmid.
(iii)
It will not be possible to differentiate transformants from the non-
transformants or to differentiate recombinants from non-recombinants.
(iv)
(b)
(i) Meloidegyne incognitia
(ii)
Agrobacterium tumefaciens / Ti Plasmid
(iii)
Both sense and antisense RNA are complementary to each other, form a
double stranded RNA (dsRNA)
(iv)
The double stranded RNA binds to a specific mRNA / initiate RNAi ,
Prevents translation of mRNA / silencing of specific mRNA of parasite or nematode.
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CBSE Class 12 Biology past-paper question from the 2024board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.