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Biology · 2025 · 2 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology2 marksUnderstandvery short answer
CBSE 2025 · Region 4 · Set 1 · Q20
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarized below in the form of a flow diagram.…
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarized below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow. Step-$\displaystyle 1$ Vector DNA (cut using + Alien DNA (cut using Restriction Enzyme EcoR I) Restriction Enzyme EcoR I) ↓ Enzyme A Step-$\displaystyle 2$ Recombinant DNA molecule ↓ Step-$\displaystyle 3$ Transfer of recombinant DNA molecule in E. coli (Host) ↓ Step-$\displaystyle 4$ Replication of recombinant DNA molecule in E. coli
(a)
Name the enzyme used in Step-$\displaystyle 1$ to join the cut plasmid and alien DNA.
(b)
State the technical term used for Step-3.
(c)
Justify the use of same Restriction Enzyme EcoR I to cut both the vector DNA and the alien DNA.
Marking-scheme solution
(a)
DNA ligase
(b)
Transformation
(c)
Same restriction EcoRI is used to cut both the vector DNA and
alien DNA because it will produce complementary overhangs or sticky ends which will help in joining two DNA strands through hydrogen bonds.
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CBSE Class 12 Biology past-paper question from the 2025 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.