CBSE 2025 · Region 6 · Set 2 · Q20 · 2 marks
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow.
(a)What is the technical term used for Step $\displaystyle 4$ in the above process ?(b)Which of the given two combinations of restriction enzyme should be used in Step $\displaystyle 1$ ? Justify your answer.(i)EcoR I to cut the plasmid and Hind III to cut the alien DNA.(ii)EcoR I to cut both the plasmid and alien DNA.
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow.
(a)
What is the technical term used for Step $\displaystyle 4$ in the above process ?
(b)
Which of the given two combinations of restriction enzyme should be used in Step $\displaystyle 1$ ? Justify your answer.
(i)
EcoR I to cut the plasmid and Hind III to cut the alien DNA.
(ii)
EcoR I to cut both the plasmid and alien DNA.
Marking-scheme solution
(a)
Gene cloning /Amplification
(b)
EcoR I to cut both the plasmid and alien DNA
Cutting with Same restriction enzyme will yield sticky ends at the end of both DNA which can be joined together by DNA ligase.
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA TechnologyApplyvery_short_answermedium
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