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Biology · 2025 · 2 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology2 marksApplyvery short answer
CBSE 2025 · Region 6 · Set 2 · Q20
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram.…
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow.
(a)
What is the technical term used for Step $\displaystyle 4$ in the above process ?
(b)
Which of the given two combinations of restriction enzyme should be used in Step $\displaystyle 1$ ? Justify your answer.
(i)
EcoR I to cut the plasmid and Hind III to cut the alien DNA.
(ii)
EcoR I to cut both the plasmid and alien DNA.
Marking-scheme solution
(a)
Gene cloning /Amplification
(b)
EcoR I to cut both the plasmid and alien DNA
Cutting with Same restriction enzyme will yield sticky ends at the end of both DNA which can be joined together by DNA ligase.
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CBSE Class 12 Biology past-paper question from the 2025 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.