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Biology · 2025 · 3 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology3 marksUnderstandshort answer
CBSE 2025 · Region 1 · Set 2 · Q22
(a) Explain the importance of 'ori' and 'rop' in the E. coli cloning vector shown in the above given diagram. (b) Differentiate between exonucleases…
(a)
Explain the importance of 'ori' and 'rop' in the E. coli cloning vector shown in the above given diagram.
(b)
Differentiate between exonucleases and endonucleases.
Marking-scheme solution
(a)
'ori' -- This is a sequence in cloning vector from where replication starts and is also responsible for controlling the copy number of the linked DNA.
'rop' -- codes for the proteins involved in the replication of the plasmids.
(b)
| Exonucleases | Endonucleases |
| It is an enzyme that cuts DNA from $\displaystyle 5$' or $\displaystyle 3$' end or terminal region of nucleic acid / It removes nucleotide from the ends of the DNA | It is an enzyme that cuts DNA from any point inside the strand / It makes cuts at specific position within the DNA. |
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