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Biology · 2025 · 3 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology3 marksApplyshort answer
CBSE 2025 · Region 1 · Set 1 · Q22
(a) Write the palindromic nucleotide sequence for following sequence of DNA segment: 5' - GAATTC - 3' (b) Name the restriction endonuclease that…
(a)
Write the palindromic nucleotide sequence for following sequence of DNA segment : $\displaystyle 5$' - GAATTC - $\displaystyle 3$'
(b)
Name the restriction endonuclease that recognizes this sequence.
(c)
How are sticky ends produced ? Mention their role.
Marking-scheme solution
(a)
$\displaystyle 3$' - CTTAAG - $\displaystyle 5$'
(b)
EcoRI
(c)
-Restriction enzyme cut the strand of DNA a little away from the Centre of the palindrome site, but between the same two bases on the opposite strands. This leaves single stranded portion at the two ends known as sticky ends.
Role of sticky ends :
Sticky ends forms hydrogen bond with their complementary cut counter part/ they help injoining of vector DNA and foreign DNA during rDNA technology/ stickiness of ends facilitates the action of enzyme DNA ligase
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CBSE Class 12 Biology past-paper question from the 2025 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.