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Biology · 2025 · 3 marks
Biotechnology and its ApplicationsBiotechnological Applications in Agriculture3 marksUnderstandshort answer
CBSE 2025 · Region 7 · Set 2 · Q23
Name the process involved in the production of nematode-resistant tobacco plants, using genetic engineering. Explain the strategy adopted to develop…
Name the process involved in the production of nematode-resistant tobacco plants, using genetic engineering. Explain the strategy adopted to develop such plants.
Marking-scheme solution
• Process involved is RNA interference (RNAi)
• Using Agrobacterium vectors nematode-specific genes were
introduced into the host plant, the introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells, these two RNAs being complementary to each other formed a double stranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode leading to its death in transgenic host.
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CBSE Class 12 Biology past-paper question from the 2025 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.