CBSE 2026 · Region 4 · Set 1 · Q20 · 2 marks
Write the role of 'ori' and 'restriction' site in a cloning vector pBR322.
Marking-scheme solution
• Ori – site where replication starts/Responsible for controlling the copy number of
the linked DNA.
• Restriction site – Site of ligation of alien DNA or foreign DNA or desirable DNA to
the vector.
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CBSE Class 12 Biology past-paper question from the 2026board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.