CBSE 2026 · Region 5 · Set 3 · Q21 · 2 marks
Explain any two methods of vectorless gene transfer.Name the most commonly used bioreactor. List the advantages of the bioreactor. Also write the importance of sampling ports.
Explain any two methods of vectorless gene transfer.
Name the most commonly used bioreactor. List the advantages of the bioreactor. Also write the importance of sampling ports.
Marking-scheme solution
(a)
− Microinjection – In this the recombinant DNA is directly injected into the nucleus of an animal cell.
− Gene gun / Biolistics – In this method the cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA.
− Any other correct method
(a)
(b)
• Simple stirred-tank bioreactor.
• Advantages
− Large quantities of products can be obtained
− All optimum growth conditions are provided in it
− Any other point
• Sampling port: Small volumes of the culture can be withdrawn periodically.
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA TechnologyUnderstandvery_short_answermedium
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