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Biology · 2026 · 2 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology2 marksUnderstandvery short answer
CBSE 2026 · Region 5 · Set 3 · Q21
(a) Explain any two methods of vectorless gene transfer.
(a)
Explain any two methods of vectorless gene transfer.
OR
(b)
Name the most commonly used bioreactor. List the advantages of the bioreactor. Also write the importance of sampling ports.
Marking-scheme solution
(a)
− Microinjection – In this the recombinant DNA is directly injected into the nucleus of an animal cell.
− Gene gun / Biolistics – In this method the cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA.
− Any other correct method
(a)
(b)
• Simple stirred-tank bioreactor.
• Advantages
− Large quantities of products can be obtained
− All optimum growth conditions are provided in it
− Any other point
• Sampling port: Small volumes of the culture can be withdrawn periodically.
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CBSE Class 12 Biology past-paper question from the 2026 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.