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Biology · 2024 · 2 marks
CBSE 2024 · Region 5 · Set 1 · Q18
5^′- G^↓ A A T T C -3^′ 3^′- C T T A A_↑ G -5^′ (a) Name the restriction enzyme that recognises the given specific sequence of bases. What are such…
$\displaystyle 5^{\prime}-\mathrm{G}^{\downarrow} \mathrm{A} \mathrm{A} \mathrm{T} \mathrm{T} \mathrm{C}-3^{\prime}$ $\displaystyle 3^{\prime}-\mathrm{C} \mathrm{T} \mathrm{T} \mathrm{A} \mathrm{A}_{\uparrow} \mathrm{G}-5^{\prime}$
(a)
Name the restriction enzyme that recognises the given specific sequence of bases. What are such sequence of bases referred to as ?
(b)
What are the arrows in the given figure indicating ? Write the result obtained thereafter.
Marking-scheme solution
(a)
• Enzyme – EcoRI,
• Palindrome / palindromic nucleotide sequences.
(b)
• Indicates the site at which EcoRI makes a cut in the two strands of DNA /
restriction sites or recognition sites of EcoRI
• thereafter gives rise to “sticky ends.”
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CBSE Class 12 Biology past-paper question from the 2024 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.