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Biology · 2023 · 2 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology2 marksUnderstandvery short answer
CBSE 2023 · Region 1 · Set 2 · Q19
(a) Vectors are DNA molecules that can carry a foreign DNA segment into the host cell. (i) Write the significance of 'ori' in this vector. (ii) Give…
(a)
Vectors are DNA molecules that can carry a foreign DNA segment into the host cell.
(i)
Write the significance of 'ori' in this vector.
(ii)
Give one example each of vectors used for cloning genes in plants and animals.
OR
(b)
Write how can an alien DNA be introduced into
(i)
an animal cell
(ii)
a plant cell.
Marking-scheme solution
(i)
'ori' - a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells, this sequences is also responsible for controlling the copy number of the linked DNA
(ii)
Plants - Agrobacterium tumefaciens Animals - Retrovirus
(i)
Micro injection, Recombinant DNA is directly injected in the nucleus of an animal cell
// Retrovirus, animal cells are infected with disarmed retrovirus
(ii)
Biolistics or Genegun, Plants cell are bombarded with high velocity micro particles of gold or tungsten coated with DNA (rDNA)
// Agrobacterium tumefaciens, delivering gene by disarmed pathogen
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CBSE Class 12 Biology past-paper question from the 2023 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.