CBSE 2025 · Region 5 · Set 2 · Q17 · 2 marks
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow:
(a)Name the specific enzyme that might have been used to make the multiple copies of foreign DNA before undergoing Step-$\displaystyle 1$ of the process.(b)How does the use of restriction enzyme EcoR I in Step-$\displaystyle 1$ facilitate the action of DNA ligase to form the recombinant DNA molecule ? Explain.(c)Name the most commonly used host in the above process.
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow:
(a)
Name the specific enzyme that might have been used to make the multiple copies of foreign DNA before undergoing Step-$\displaystyle 1$ of the process.
(b)
How does the use of restriction enzyme EcoR I in Step-$\displaystyle 1$ facilitate the action of DNA ligase to form the recombinant DNA molecule ? Explain.
(c)
Name the most commonly used host in the above process.
Marking-scheme solution
(a)
Taq polymerase
(b)
It forms overhanging stretches called sticky ends, which facilitates hydrogen bond
formation with their complementary cut parts by DNA ligase.
(c)
Escherichia coli/E.coli
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA TechnologyUnderstandvery_short_answermedium
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