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Biology · 2025 · 2 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology2 marksUnderstandvery short answer
CBSE 2025 · Region 5 · Set 2 · Q17
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram.…
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow:
(a)
Name the specific enzyme that might have been used to make the multiple copies of foreign DNA before undergoing Step-$\displaystyle 1$ of the process.
(b)
How does the use of restriction enzyme EcoR I in Step-$\displaystyle 1$ facilitate the action of DNA ligase to form the recombinant DNA molecule ? Explain.
(c)
Name the most commonly used host in the above process.
Marking-scheme solution
(a)
Taq polymerase
(b)
It forms overhanging stretches called sticky ends, which facilitates hydrogen bond
formation with their complementary cut parts by DNA ligase.
(c)
Escherichia coli/E.coli
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