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Biology · 2022 · 3 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology3 marksRemembershort answer
CBSE 2022 · Region 1 · Set 3 · Q11
Name any three techniques used for introducing an alien DNA into a host cell and mention how?
Name any three techniques used for introducing an alien DNA into a host cell and mention how ?
Marking-scheme solution
-Microinjection-recombinant DNA is directly injected into the nucleus of an animal cell.
-Biolistics or gene gun-Host cells are bombarded with high velocity micro- particles of gold or tungsten coated with DNA.
-Disarmed pathogen vectors -transfers the recombinant DNA into the host cells when allowed to infect
-Heat shock method-recombinant DNA is forced into competent bacterial cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at $\displaystyle 42$ degree Celsius (heat shock) and then putting them back on ice.
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CBSE Class 12 Biology past-paper question from the 2022 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.