(a) Explain how the process of RNA interference technology is used effectively to prevent infestation of the roots of tobacco plant by the nematode…

Using Agrobacterium vectors nematode–specific genes were introduced into the

host plants, the introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells, these two RNA’s being complementary to each other formed a double stranded (dsRNA), that initiated RNAi and thus silenced the specific mRNA of the nematode, consequently the parasite could not survive in a transgenics host expressing specific interfering RNA

‘in vitro’ using two sets of primers

isolated from a bacterium Thermus aquaticus, extends the primers using the deoxyribonucleotides provided in the reaction and the genomic DNA as a the template

billion copies of a fragment of DNA