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Biology · 2025 · 3 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology3 marksUnderstandshort answer
CBSE 2025 · Region 1 · Set 3 · Q28
(A) Differentiate between Recombinant DNA and cDNA. (B) Explain the method to increase the competency of the bacterial cell membrane to take up…
(A)
Differentiate between Recombinant DNA and cDNA.
(B)
Explain the method to increase the competency of the bacterial cell membrane to take up recombinant DNA.
Marking-scheme solution
(a)
Recombinant DNA is formed by joining together two DNA fragments from two different sources. cDNA or complimentary DNA is formed by the reverse transcription of mRNAs.
(b)
After treatment with divalent cation such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall, then incubated the cell on ice, followed by placing them briefly at $\displaystyle 42$°C (heat shock) and then putting them back on ice.
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CBSE Class 12 Biology past-paper question from the 2025 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.