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Biology · 2022 · 3 marks
Biotechnology : Principles and ProcessesProcesses of Recombinant DNA Technology3 marksRemembershort answer
CBSE 2022 · Region 4 · Set 1 · Q12
Causative agents of HIV-AIDS and COVID-19 belong to the same group of viruses. To diagnose and amplify the genetic material for further study of…
Causative agents of HIV-AIDS and COVID-$\displaystyle 19$ belong to the same group of viruses. To diagnose and amplify the genetic material for further study of COVID-$\displaystyle 19$ virus, 'RT-PCR' test is carried out.
(a)
What does 'RT-PCR' stand for ?
(b)
Explain the various steps of PCR technique.
Marking-scheme solution
(a) Real Time-Polymerase Chain Reaction / Reverse Transcriptase- Polymerase Chain Reaction
(b) • Denaturation by heating/DNA strands are separated by heating, Annealing of two primers to complementary region of DNA/ Joining of primer to complementary region of DNA, Extension of primers, using thermostable DNA Polymerase or Taq Polymerase
(The process is repeated many times/amplification)
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CBSE Class 12 Biology past-paper question from the 2022 board exam, with the answer as CBSE’s own marking scheme gives it. Where our answers come from.