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Biology · 2024 · 5 marks
CBSE 2024 · Region 5 · Set 3 · Q33
(a) Work out a dihybrid cross upto F_2 generation between pea plants bearing violet coloured axial flowers and white coloured terminal flowers using…
(a)
Work out a dihybrid cross upto $\displaystyle \mathrm{F}_{2}$ generation between pea plants bearing violet coloured axial flowers and white coloured terminal flowers using Punnet's square. Give their $\displaystyle \mathrm{F}_{2}$ phenotypic ratio. State the Mendel's law of inheritance that was derived from such a cross.
OR
(b)
Explain the process of transcription in prokaryotes. How is it different from transcription in eukaryotes ?
Marking-scheme solution
Violet and axial : Violet and terminal : White and axial : White and terminal
$\displaystyle 9$ : $\displaystyle 3$ : $\displaystyle 3$ : $\displaystyle 1$
Mendel’s Law of Independent Assortment, when two pairs of traits are combined in a hybrid segregation of one pair of character is independent of the other pair of characters.
DNA dependent RNA polymerase binds to promoter and initiate transcription on a DNA template, it uses nucleoside tri-phosphate as a substrate and polymerizes in a template dependent fashion following the rule of complementarity, RNA polymerase associates with the initiation/sigma/σ factor(transiently), facilitate opening of the helix, and continue elongation, once the polymerase reaches terminator region, it binds to rho/ρ/termination factor and the nascent RNA falls off, RNA polymerase also falls off once the termination is over.
Transcription
in
Prokaryotes
Transcription
in
Eukaryotes
One type of RNA
polymerase
catalyses
transcription of all $\displaystyle 3$
types of RNA.
Three different RNA
polymerases
are
required for different
$\displaystyle 3$ types of RNA
(RNA
Polymerase–I
rRNA
RNA
Polymerase–II
mRNA
RNA
Polymerase–III
tRNA)
No splicing required
as only exons are
present
splicing is required as
both
introns
and
exons are present
No
processing
of
RNA is required as
hnRNA
is
not
formed
Processing
(capping,
tailing and splicing)
required as hnRNA is
initially formed
It occurs in cytoplasm
of the cell
It occurs in nucleus of
the cell
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